murine normal liver cell line aml12  (ATCC)

Journal: Cell reports

Article Title: Menin-MLL1 complex cooperates with NF-Y to promote hepatocellular carcinoma survival

doi: 10.1016/j.celrep.2025.116619

Figure Lengend Snippet: (A) Scheme of epigenome-focused CRISPR-Cas9 screening in 3D settings in the presence of SNDX-5613 or DMSO. (B) Heatmap of potential menin inhibitor combinatorial targets from the CRISPR-Cas9 screen performed in HLF cells treated with 1 μM SNDX-5613 or DMSO for 28 days. The score was calculated as a subtraction of SNDX-5613 from DMSO at days 28/0. NFYB is shown in red. (C) Comparison of gene scores based on gene enrichment in DMSO- and SNDX-5613- (1 μM) treated groups from 3D CRISPR screens on HLF cells. (D) Summary of negative-selection CRISPR-Cas9 screen with sgRNAs targeting NFYB gene or with PCNA or Rosa26 serving as the positive or negative controls, respectively, in 2D depicted at days 3, 14, 21, and 28. Bar graphs represent the mean of measurements of 3 independently transduced sets of cells using the same lentivirus. Error bars represent the SD. (E) Immunoblotting against NF-YB and β-Actin proteins in HLF-KO and NTC cells. (F) Cell viability of HLF, PLC/PRF/5, Hep3B, THLE-2, or AML12 cells with either NFYB KO or NTC upon SNDX-5613 treatment for 7 or 10 days, as indicated in 2D, assessed by the CTG assay. Error bars represent the SD of 3 independent experiments for HLF and PLC/PRF/5, 2 for Hep3B and THLE-2, or 1 for AML12 cells, each performed in 4 technical replicates. * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, and **** p ≤ 0.0001 were calculated using a Student’s t test. (G and H) HCC and normal liver cell viability and growth after 7 or 14 days of SNDX-5613 treatment as indicated in 3D media. Cell growth and colony formation ability were assessed by microscopy (G), and cell viability was measured using the CTG assay (H). Micrographs were taken at ×1.25 amplification, with the bar indicating 1.3 mm. (I) Cell viability of HCC and normal liver cells with NFYB or NTC KO measured using the CTG assay. Bar graphs represent the mean of measurements of 3 (HLF, PLC/PRF/5, and AML12) or 2 (Hep3B) independently transduced sets of cells using the same lentivirus. Error bars represent the SD.

Article Snippet: All HCC (HLF (RRID:CVCL_2947), PLC/PRF/5 (RRID:CVCL_0485), SNU398 (RRID:CVCL_0077), and SNU449 (RRID:CVCL_0454)), murine normal liver AML12 (RRID:CVCL_0140) and Lenti-X 293 (RRID:CVCL_0045) cell lines were purchased from ATCC or were a gift as noted.

Techniques: CRISPR, Comparison, Selection, Western Blot, CTG Assay, Microscopy, Amplification

Journal: Frontiers in Pharmacology

Article Title: Methyl 6-O-cinnamoyl-α- d -glucopyranoside Ameliorates Acute Liver Injury by Inhibiting Oxidative Stress Through the Activation of Nrf2 Signaling Pathway

doi: 10.3389/fphar.2022.873938

Figure Lengend Snippet: (A) Synthetic route and structure of MCGP. (B-D) Cytotoxicity of MCGP on 7,721 (B) , THP-1 (C) , and LX-2 (D) cells at 25–1,000 μM. (E,F) Cytotoxicity of MCGP on HepG2 (E) and AML12 (F) cells at 25–400 μM. Data were presented as the mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001 vs control.

Article Snippet: Human liver cancer cell line 7,721 and HepG2, human hepatic stellate cell line LX-2, human monocytic leukemia cell line THP-1, and murine normal liver cell AML12 were purchased from Procell Life Science & Technology Co., Ltd. (Wuhan, China).

Techniques: Control

Journal: Frontiers in Pharmacology

Article Title: Methyl 6-O-cinnamoyl-α- d -glucopyranoside Ameliorates Acute Liver Injury by Inhibiting Oxidative Stress Through the Activation of Nrf2 Signaling Pathway

doi: 10.3389/fphar.2022.873938

Figure Lengend Snippet: MCGP protected AML12 cells from APAP- or CCl 4 -induced hepatotoxicity. (A) The cell viability of AML12 cells co-treated with MCGP at 50–200 μM and 25 mM of APAP or 30 mM of CCl 4 . (B,C) Cell apoptosis of AML12 cells co-treated with MCGP at 50–200 μM and 25 mM of APAP (B) or 30 mM of CCl 4 (C) . (D) The ROS generation of MCGP at 50–200 μM on AML12 cells stimulated by 400 μM of H 2 O 2 . (E) The inhibition rates of ABTS •+ of MCGP and ascorbic acid at 25–1,000 μM. Data were presented as the mean ± SEM. # p < 0.05, ## p < 0.01, ### p < 0.001 vs control, * p < 0.05, ** p < 0.01 vs APAP or CCl 4 .

Article Snippet: Human liver cancer cell line 7,721 and HepG2, human hepatic stellate cell line LX-2, human monocytic leukemia cell line THP-1, and murine normal liver cell AML12 were purchased from Procell Life Science & Technology Co., Ltd. (Wuhan, China).

Techniques: Inhibition, Control